Platisil™ NH2 (New)

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Platisil NH2 columns retain hydrogen-bonding compounds under three separation modes – RP, NP, and IC. Platisil NH2 columns provide reproducible retention and selectivity with improved column lifetime.

 

Features of Platisil™ NH2 Columns

·  Aminopropyl modified silica phase for multi-mode chromatography (RP, NP, &IC)
·  Improved phase ruggedness and stability
·  Excellent retention for polar compounds such as sugars, oligosaccharides, sugar alcohols, and other hydroxyl compounds, as well as DNA bases under RP conditions and vitamins A & D and hydrocarbons in the petroleum industry under NP conditions

 

Platisil™ NH2 Material Characteristics

Bonded phase

Particle size(μm)

Pore size(Å)

Surface area(m2/g)

Purity(%)

Phase density(µmol/m2)

Carbon loading(%)

pH range

Endcapping

NH2

3, 5, 10

100

440

>99.999

3.2

7

2.0-7.5

No

 

 

 

 

Sugars
Column: PlatisilTM NH2   250 × 4.6 mm, 5 µm
Cat. No.: 99505
Mobile Phase: MeCN:H2O= 75:25
Flow Rate: 1.0 mL/min
Detection: RI
Temperature: 40 ºC
Sample: 1. Fructose                       4. Maltose
  2. Glucose                        5. Lactose
  3. Sucrose

  

Sugar alcohols
Column: PlatisilTM NH2   250 × 4.6 mm, 5 µm
Cat. No.: 99505
Mobile Phase: MeCN:H2O= 85:15
Flow Rate: 1.0 mL/min
Detection: RI
Temperature: 40 ºC
Sample: 1. meso-Erythritol                      4. Sorbitol
  2. Xylitol                                        5. Mannitol
  3. Fructose

 

Malto oligosugars
Column: PlatisilTM NH2   250 × 4.6 mm, 5 µm
Cat. No.: 99505
Mobile Phase: MeCN:H2O= 55:45
Flow Rate: 1.0 mL/min
Detection: RI
Temperature: 40 ºC
Sample: 1. Maltose                        4. Maltopentaose
  2. Maltotriose                 5. Maltoheptaose
  3. Maltotetraose

 

Nucleic acid bases
Column: Listed on chromatograms  
Dimension: 150 × 4.6 mm, 5 µm
Mobile Phase: MeCN:20 mM ammonium formate= 80:20
Flow Rate: 1.0 mL/min
Detection: UV 254 nm
Temperature: Ambient
Sample: 1. Thymine                 3. Adenine
  2. Uracil                      4. Cytosine

Dikma Platisil NH2 (Cat#: 99504)

Agilent Polaris NH2

 

Water-soluble vitamins
Column: Listed on chromatograms  
Dimension: 150 × 4.6 mm, 5 µm
Mobile Phase: MeCN:25 mM KH2PO4 (pH=2.5) = 70:30
Flow Rate: 1.0 mL/min
Detection: UV 254 nm
Temperature: Ambient
Sample: 1. Riboflavin                            3. Pyridoxine
  2. Nicotinamide                     4. Thiamine

Dikma Platisil NH2  (Cat#: 99504)

GL Inertsil NH2 

Phenomenex Luna NH2

  

Salicylic acids
Column: Listed on chromatograms  
Dimension: 150 × 4.6 mm, 5 µm
Mobile Phase: MeCN:20 mM CH3COONH4 (pH=6.8) = 80:20
Flow Rate: 1.0 mL/min
Detection: UV 228 nm
Temperature: Ambient
Sample: 1. Salicylamide                           3. 4-Aminosalicylic acid
  2. Salicylic acid                           4. Acetylsalicylic acid

Dikma Platisil NH2  (Cat#: 99504)

GL Inertsil NH2 

Phenomenex Luna NH2

 

  

Steroids
Column: Listed on chromatograms    
Dimension: 150 × 4.6 mm, 5 µm  
Mobile Phase: Hexane:Ethanol = 90:10  
Flow Rate: 2.0 mL/min  
Detection: UV 254 nm  
Temperature: Ambient  
Sample: 1. 11-Ketoprogesterone  6. Cortisone
  2. Progesterone  7. Prednisone
  3. Cortisone 21-acetate 8. Hydrocortisone
  4. Prednisolone 21-acetate  9. Prednisolone
  5. Corticosterone  10. Dexamethasone

Dikma Platisil NH2  (Cat#: 99504)

GL Inertsil NH2 

Phenomenex Luna NH2

 

 

 

 

 

 

 

 


 

EasyGuard™ Columns

• Universal design to match any brand column
• A variety of optional bonded phase material
• Does not affect the analytical column resolution
• Long lifetime column cartridges, high performance and low price

 

Balance

Guard columns provide protection against contamination with minimal impact on column efficiency. The column diameter determines both the sample loadability and column efficiency. A small diameter will decrease the column lifetime, but a large diameter will adversely affect column resolution. EasyGuard™ columns effectively profect the analytical column without adversely affecting the resolution or column lifetime.

Simple to Use

• Flexibility to move PEEK fittings
• Matches any brand analytical column
• Low dead volume connection
• Rugged 316 stainless steel column holder

 

Easy-Lok™ Coupler New!

 

When the EasyGuard™ guard column connects to a 250 mm analytical column, it can not fit in certain ovens (such as Agilent 1200) because the length is not long enough. The Easy-Lok™ coupler resolves this issue by removing the pre-tightened connecting pipe by wrench before using.