Platisil CN columns offer a unique polar selectivity in RP and NP mode. They provide sharp peaks and great reproducibility run-to-run, column-to-column, and batch-to-batch. The smooth silica allows for a more uniform bonding with improved resistance to bonded phase hydrolysis to produce one of the most stable CN phases. The high coverage combined with a thorough endcapping makes Platisil CN columns suitable for the separation of ionizable compounds such as basic drugs, organic acids, and steroids as well as carboxyl, carbonyl, and amine containing compounds.

 

Features of Platisil™ CN Columns      

  

·     Monomerically bonded cyanopropyl group

·     Exceptionally high surface coverage and alternative selectivity

·     Low hydrophobicity for rapid elution of hydrophobic analytes

·     More reproducible separations than silica for NP applications

·     Multi-mode column (RP, NP, & HILIC) widens scope of selectivity

·     High retention capacity for polar and unsaturated compounds

·     Excellent reproducibility and superior stability

·     Quick equilibration and less sensitivity to small changes of the water content in the mobile phase

·     Suitable for the separation of ionizable compounds such as basic drugs, organic acids, and steroids

 

 Platisil™ CN Material Characteristics

Bonded phase	Particle size(μm)	Pore size(Å)	Surface area(m2/g)	Purity(%)	Phase density(µmol/m2)	Carbon loading(%)	pH range	Endcapping
CN	3, 5, 10	100	440	>99.999	4.8	12	1.5-7.5	Yes

 

 

   Batch-to-batch reproducibility of Platisil CN: SelectivityBatch-to-batch reproducibility of Platisil CN: SelectivityBatch-to-batch reproducibility of Platisil CN: SelectivityBatch-to-batch reproducibility of Platisil CN: Selectivi Batch-to-batch reproducibility of Platisil CN: Selectivity

 

  

 Batch-to-batch reproducibility of Platisil CN: Capacity factor

 

 

Platisil™ CN Material Characteristi

Platisil™ CN Material Characteristics

Platisil™ CN Material Characteristi

 

 

 

 

 

 

Selectivity and retention comparison

Column: Listed on chromatograms     Dimension: 150 × 4.6 mm, 5 µm   Mobile Phase: MeOH:H2O = 65:35   Flow Rate: 1.0 mL/min   Detection: UV 254 nm   Temperature: Ambient   Sample: 1. Uracil                           5. Butylbenzene   2. Caffeine                       6. Amylbenzene   3. Phenol                        7. o -Terphenyl   4. Toluene                       8. Triphenylene

Dikma Platisil CN (Cat#: 99506) Dikma Inspire C18 (Cat#: 81001)

 

Phthalate esters
Normal Phase     Reversed Phase
Column: Platisil CN, 150 × 4.6 mm, 5 µm Column: Platisil CN, 150 × 4.6 mm, 5 µm Cat. No.: 99506 Cat. No.: 99506 Mobile Phase: Hexane:Ethanol = 95:5 Mobile Phase: MeCN:H2O = 65:35 Flow Rate: 1.0 mL/min Flow Rate: 1.0 mL/min Detection: Ambient Detection: Ambient Temperature: UV 254 nm Temperature: UV 254 nm Sample: 1. Dioctyl Phthalate Sample: 1. Dimethyl phthalate   2. Dibutyl phthalate   2. Diethyl phthalate   3. Dipropyl phthalate   3. Dipropyl phthalate   4. Diethyl phthalate   4. Dibutyl phthalate   5. Dimethyl phthalate   5. Dioctyl Phthalate

  

Anti-inflammatories

Column: Listed on chromatograms   Dimension: 150 × 4.6 mm, 5 µm Mobile Phase: MeCN+0.1% HCOOH:H2O+0.1% HCOOH = 50:50 Flow Rate: 1.0 mL/min Detection: UV 254 nm Temperature: Ambient Sample: 1. Phenacetin                         4. Ibuprofen   2. Tolmetin                             5. Diclofenac   3. Ketoprofen

Dikma Platisil CN (Cat#: 99506)   GL Inertsil CN-3 Phenomenex Luna CN

 

 

Steroids

Column: Listed on chromatograms     Dimension: 150 × 4.6 mm, 5 µm   Mobile Phase: Hexane:Ethanol = 90:10   Flow Rate: 2.0 mL/min   Detection: UV 254 nm   Temperature: Ambient   Sample: 1. 11-Ketoprogesterone                       4. Prednisolone   2. Prednisolone 21-acetate                 5. Cortisone   3. Corticosterone                                   6. Dexamethasone

Dikma Platisil CN (Cat#: 99506)   Phenomenex Luna CN Agilent ZORBAX SB-CN